Hct116 cells cin
WebSep 25, 2024 · The human HCT116 colon carcinoma cell line and the K562 chronic myelogenous leukemia cell linewere cultivated in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. Cells were seeded at a density of 1×10 5 into 6-well plates prior to drug treatment. WebFeb 22, 2024 · Consistent with previous work, the magnitude and variance of mitotic delays were larger in KIF18A KD CIN tumor cells than diploid (MCF10A) or near-diploid cells …
Hct116 cells cin
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WebApr 4, 2005 · To assess whether telomere shortening and anaphase bridging could actually induce MM, HCT116 cells were grown in the presence of the MST-312 telomerase … WebDec 5, 2024 · Specifically, HCT116-WT cells predominantly show epithelial-like morphology (round and organized sheet pattern), whereas HCT116-MT cells mostly display mesenchymal-like morphology...
Webcin or 5FU. MDM2 silencing by RNA interference acti-vated p53 target gene expression in normal fibroblasts but not in HCT116 cells, and exogenous p53 could be stabilized in HCT116 knockout cells despite combined mutation of p53 phosphorylation sites and silencing of MDM2 expression. The MDM2 feedback loop is thus de- WebCCL-247EMT ™. HCT116 VIM RFP is a cell line exhibiting epithelial morphology that was isolated in 2024 from the rectum of a male patient with carcinoma colorectal. This cell line was deposited by MG Brattain and can be used in epithelial to mesenchymal transition (EMT), anti-EMT drug screening, colon cancer drug screening, vimentin ...
WebCarcinoma in situ (Intraepithelial neoplasia, CIN) is present in about 85% of sporadic colorectal cancers. Carcinogenesis is caused by defects in telomere ... HCT116 cells with caspase-3 gene knockout showed increased sensitivity to chemotherapy and radiotherapy, and weakened tumor invasiveness and metastasis ability, suggesting that ... WebFeb 18, 2008 · We selected two chromosomally stable cell lines (HCT116 and RPE-1) and three aneuploid and CIN cell lines (HT29, MCF-7, and Caco2) for in-depth analyses in …
WebHCT116-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1 binding sites. HCT116-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in ...
WebAug 8, 2014 · We verified by PCR however, the HCT116 p53-/- still express all the p53 isoform (D40p53alpha,beta,gamma), d133p53alpha,beta,gamma and also the D160p53alphabeta and gamma) The p53 protein isoforms... daytona beach vacation rentals by owner houseWebJan 27, 2024 · We used single-cell DNA sequencing 41 to karyotype a total of 210 single cells across two near-diploid (HCT116-WT and HCT116-GFP) and two highly aneuploid … daytona beach vacation rental companiesWebHCT116 Organism Human Passage number 20 Biosafety level 1 General notes Wild-type cell lines are sold with knockout cell lines only - not available for individual purchase. … gdb show assembly at addressWebCCL-247-LUC2 ™. HCT 116-Luc2 is a cell line exhibiting epithelial morphology that was isolated in 2024 from the colon of an adult male patient with carcinoma colorectal. This cell line can be used in assay development, and cancer research. The cells constitutively express high levels of enzymatically active luciferase protein, which can be ... gdb show errnoWebApr 30, 2024 · HCT116 cells were obtained from the Cell Bank of the Chinese Academy of Science (Shanghai, China) and cultured in Dulbecco’s modified Eagle medium (HyClone, … daytona beach vacation rentals cheapWebDisclosed is a method for increasing susceptibility of cancer cells to ionizing radiation by delivering to the cells a radiosensitizing agent that has one of the following properties: it perturbs the process of chromosome segregation thereby increasing chromosome missegregation; or (b) it is an inhibitor of an agent that promotes faithful chromosome … gdb show current thread idWebThe findings indicated that HCT-116 cells were not significantly sensitive to ColN but full length ColN caused slight decrease in cancer cell viability. The data in this study will … gdb show current source line